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interactome:bac96culture [2019/11/30 16:19]
dan created
interactome:bac96culture [2019/11/30 16:20]
dan
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   *Adhesive aluminium seals   *Adhesive aluminium seals
   *Adhesive breathable seals   *Adhesive breathable seals
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 === Protocol === === Protocol ===
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 ==1. Sample prep == ==1. Sample prep ==
  
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   *Once thawed, spin samples at 1000 x g for 1 min.   *Once thawed, spin samples at 1000 x g for 1 min.
   *Remove seal.   *Remove seal.
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 ==2. Medium prep (Can do while waiting for sample thaws)== ==2. Medium prep (Can do while waiting for sample thaws)==
    
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     DHFR3-96G006     DHFR3-96G006
     DHFR3-96G010     DHFR3-96G010
 +    ​
   *With a liquidator, aliquiot 1000µL of medium to each well (200 µL x 5).   *With a liquidator, aliquiot 1000µL of medium to each well (200 µL x 5).
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 ==3. Inoculation== ==3. Inoculation==
  
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   *Culture sample at 37˚C, 160 rpm (circular rotation) for 2 O/N.   *Culture sample at 37˚C, 160 rpm (circular rotation) for 2 O/N.
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  --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/30 16:11//  --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/30 16:11//
   
  • interactome/bac96culture.txt
  • Last modified: 2019/11/30 16:20
  • by dan