Experiments and protocol conducted by Dan
Personal Interactome
20191122 | Personal Interactome | RNA extraction & cDNA synthesis
20191123 | Personal Interactome | Target gene amplification from cDNA
20191124 | Personal Interactome | Target gene amplification from cDNA
20191125 | Personal Interactome | Target gene amplification from cDNA
20191125 | Personal Interactome | HEK293T Entry clone generation
20191125 | Personal Interactome | Target gene amplification from cDNA
20191127 | Personal Interactome | pENTRY insert check PCR
20191210 | Personal Interactome | Target gene amplification from cDNA
20191219 | Personal Interactome | Target gene amplification from cDNA
Barcoded cDNA synthesis
20191129 | Personal Interactome | Barcoded primer generation
20191211 | Personal Interactome | Barcoded primer purification
20191210 | Personal Interactome | Barcoded target gene amplification from cDNA
Barcoded cDNA synthesis from barcode library
20191220 | Personal Interactome | Barcoded primer generation from barcoded library
20191226 | Personal Interactome | Barcoded primer generation | Biotin PCR
BP cloning
POEM project
20191122 | N-term DHFR plasmid construction | Insert generation
20191123 | N-term DHFR plasmid construction | Insert generation
20191124 | N-term DHFR plasmid construction | T4 ligation
20191127 | N-term DHFR plasmid construction | T4 ligation
20191129 | N-term DHFR plasmid construction | RE check
20191129 | N-term DHFR plasmid construction | Insert generation
20191129 | N-term DHFR plasmid construction | T4 ligation
20191211 | N-term DHFR | Pilot screening | Yeast transformation
20200329 | TriZol, column RNA extraction (DNaseI), cDNA amplification