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interactome:experiments:20191122-2 [2019/11/22 17:53] dan |
interactome:experiments:20191122-2 [2019/11/22 17:58] (current) dan |
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| ## RNA extraction from HEK293T cells | ## RNA extraction from HEK293T cells | ||
| - | {{tag>protocol RNA kit interactome PersonalInteractome}} | + | {{tag>interactome PersonalInteractome}} |
| #### Material | #### Material | ||
| Line 151: | Line 151: | ||
| ## 1st strand cDNA synthesis of RNA samples extracted on 20191122 | ## 1st strand cDNA synthesis of RNA samples extracted on 20191122 | ||
| - | {{tag>protocol RNA cDNA kit interactome PersonalInteractome}} | ||
| #### Material | #### Material | ||
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| ^ Total ^ 10 ^ 100 | | | ^ Total ^ 10 ^ 100 | | | ||
| + | *Aliquot to 10 x 10 µL | ||
| *Incubate at 65˚C for 5 mins | *Incubate at 65˚C for 5 mins | ||
| *Transfer tube to ice immediately | *Transfer tube to ice immediately | ||
| Line 221: | Line 220: | ||
| *The cDNA product should be stored at -20°C. | *The cDNA product should be stored at -20°C. | ||
| *For downsteam PCR amplification, the volume of cDNA product should not exceed 1/10 of the PCR reaction volume. | *For downsteam PCR amplification, the volume of cDNA product should not exceed 1/10 of the PCR reaction volume. | ||
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| + | ^ Sample No. ^ Date prepared ^ Number of freeze ^ Number of thaw ^ "Done" if used ^ | ||
| + | | 1 | 20191122 | 1 | 0 | | | ||
| + | | 2 | 20191122 | 1 | 0 | | | ||
| + | | 3 | 20191122 | 1 | 0 | | | ||
| + | | 4 | 20191122 | 1 | 0 | | | ||
| + | | 5 | 20191122 | 1 | 0 | | | ||
| + | | 6 | 20191122 | 1 | 0 | | | ||
| + | | 7 | 20191122 | 1 | 0 | | | ||
| + | | 8 | 20191122 | 1 | 0 | | | ||
| + | | 9 | 20191122 | 1 | 0 | | | ||
| + | | 10 | 20191122 | 1 | 0 | | | ||
| \\ | \\ | ||
| \\ | \\ | ||
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| ==Protocol last updated== | ==Protocol last updated== | ||
| - | --- //[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/21 15:16// | + | --- //[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/22 17:55// |
| - | \\ | + | |
| - | \\ | + | |
| - | ==Protocol used== | + | |