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interactome:experiments:20191122-2 [2019/11/22 17:55]
dan
interactome:experiments:20191122-2 [2019/11/22 17:58] (current)
dan
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 ## RNA extraction from HEK293T cells ## RNA extraction from HEK293T cells
-{{tag>protocol RNA kit interactome PersonalInteractome}}+{{tag>​interactome PersonalInteractome}}
  
 #### Material #### Material
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 ## 1st strand cDNA synthesis of RNA samples extracted on 20191122 ## 1st strand cDNA synthesis of RNA samples extracted on 20191122
-{{tag>​protocol RNA cDNA kit interactome PersonalInteractome}} 
  
 #### Material #### Material
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   *The cDNA product should be stored at -20°C. ​   *The cDNA product should be stored at -20°C. ​
   *For downsteam PCR amplification,​ the volume of cDNA product should not exceed 1/10 of the PCR reaction volume.   *For downsteam PCR amplification,​ the volume of cDNA product should not exceed 1/10 of the PCR reaction volume.
 +
 +
 +^  Sample No.  ^  Date prepared ​ ^  Number of freeze ​ ^  Number of thaw  ^  "​Done"​ if used  ^
 +|  1           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  2           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  3           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  4           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  5           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  6           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  7           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  8           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  9           ​| ​ 20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 +|  10          |  20191122 ​      ​| ​ 1                 ​| ​ 0               ​| ​                 |
 \\ \\
 \\  \\
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 \\  \\
 ==Protocol last updated== ==Protocol last updated==
- --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/21 15:16// + --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/11/22 17:55//
-\\ +
-\\  +
-==Protocol used==+
  • interactome/experiments/20191122-2.1574412906.txt.gz
  • Last modified: 2019/11/22 17:55
  • by dan