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| - | ## Passaging HEK293 Cells (Protocol from Arman Adel,Dec 2nd, 2019) | + | ## Passaging HEK293 Cells |
| + | (Protocol from Arman Adel,Dec 2nd, 2019) | ||
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| ==Reagents:== | ==Reagents:== | ||
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| *0.05% w/v Trypsin (0.53 mmol/l EDTA-4NA with Phenol Red) | *0.05% w/v Trypsin (0.53 mmol/l EDTA-4NA with Phenol Red) | ||
| *DMEM High Glucose (10% FBS, 1% Pen-strep) | *DMEM High Glucose (10% FBS, 1% Pen-strep) | ||
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| + | ==Preparation:== | ||
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| + | *Pre-warm the reagents to 37˚C using the water tbath. | ||
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| ==Method:== | ==Method:== | ||
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| *Add 10ml of DMEM to 10cm dish(es) with passaged cells | *Add 10ml of DMEM to 10cm dish(es) with passaged cells | ||
| *Place dish(es) in 37˚C cell culture incubators | *Place dish(es) in 37˚C cell culture incubators | ||
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| --- //[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/12/02 20:38// | --- //[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/12/02 20:38// | ||