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interactome:hek393t_passage [2019/12/02 20:39]
dan created
interactome:hek393t_passage [2019/12/02 20:42] (current)
dan
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-## Passaging HEK293 Cells (Protocol from Arman Adel,Dec 2nd, 2019) +## Passaging HEK293 Cells  
 +(Protocol from Arman Adel,Dec 2nd, 2019)
  
 +\\
 +\\
  
 ==Reagents:​== ==Reagents:​==
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   *0.05% w/v Trypsin (0.53 mmol/l EDTA-4NA with Phenol Red)    *0.05% w/v Trypsin (0.53 mmol/l EDTA-4NA with Phenol Red) 
   *DMEM High Glucose (10% FBS, 1% Pen-strep)   *DMEM High Glucose (10% FBS, 1% Pen-strep)
 +\\
 +\\
 +
 +==Preparation:​==
 +
 +  *Pre-warm the reagents to 37˚C using the water tbath.
 +\\
 +\\
  
 ==Method:== ==Method:==
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   *Add 10ml of DMEM to 10cm dish(es) with passaged cells   *Add 10ml of DMEM to 10cm dish(es) with passaged cells
   *Place dish(es) in 37˚C cell culture incubators   *Place dish(es) in 37˚C cell culture incubators
 +\\ 
 +\\ 
 +\\ 
 +\\
  --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/12/02 20:38//  --- //​[[daney@sfc.keio.ac.jp|Dan Yamamoto-Evans]] 2019/12/02 20:38//
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  • Last modified: 2019/12/02 20:39
  • by dan