Protocol for RE digestion
Material
- Sample : Total RNA sample
- PrimeScript™ II 1st strand cDNA Synthesis Kit (TaKara, Cat.6210A)
PrimeScript™ II 1st strand cDNA Synthesis Kit (For 50 reactions)
Components | Volume | Storage | Memo |
---|---|---|---|
PrimeScript II RTase(200 U/μl) | 50 μl | -20°C | |
5×PrimeScript II Buffer | 200 μl | -20°C | |
RNase Inhibitor(40 U/μl) | 25 μl | -20°C | |
dNTP Mixture (10 mM each) | 50 μl | -20°C | |
Oligo dT Primer(50 μM) | 50 μl | -20°C | |
Random 6 mers(50 μM) | 100 μl | -20°C | |
RNase free dH2O | 1 ml | -20°C |