Based on the user guide of Trizol reagent by Invitrogen.

Material

  • Sample : 6 x 10^6 of cell-line cells OR 20 mg of Tissue sample
Contents Cat. No. 15596026 (100 reactions) Cat. No. 15596018 (200 reactions) Storage
TRIzolTM Reagent 100 mL 200 mL 15–30°C

=Required items which is not supplied with the kit.=

Item Memo
Equipment
Water bath or heat block at 55–60°C
Reagents
Isopropanol
Ethanol, 75%
RNase-free water of 0.5% SDS
(Optional) RNase-free glycogen
  • Freshly prepared DNaseI solution included in the kit
Check Reagent 1 reaction (µL) _ _ _ _ reaction (µL) Memo
ddH2O (RNAse free) 87.5
10x DNase Buffer 10
DNaseI 2.5 12 units / µL
Total volume (µL) 100 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _









Protocol

1 Sample preparation
First step of sample preparation differs if you are using (A)culture cells OR (B)Tissue specimen. 
Buffers are highlighted in blue to prevent mistakes.
  • interactome/trizol.1575512408.txt.gz
  • Last modified: 2019/12/05 11:20
  • by dan