Based on the user guide of Trizol reagent by Invitrogen.

Material

Contents and storage
Contents Cat. No. 15596026 (100 reactions) Cat. No. 15596018 (200 reactions) Storage
TRIzolTM Reagent 100 mL 200 mL 15–30°C
Required items which is not supplied.
Item Memo
Equipment
Water bath or heat block at 55–60°C
Reagents
Isopropanol
Ethanol, 75%
RNase-free water of 0.5% SDS
(Optional) RNase-free glycogen

Input sample requirements

IMPORTANT!

Perform RNA isolation immediately after sample collection or quick-freeze samples immediately after collection and store at –80°C or in liquid nitrogen until RNA isolation.
Sample type Starting material per 1 mL of TRIzolTM Reagent
Tissues[1] 50 ー 100 mg of tissue
Cells grown in monolayer 1 × 105 ー 1 × 107 cells grown in monolayer in a 3.5–cm culture dish (10 cm2)
Cells grown in suspension 5 ー 10 × 106 cells from animal, plant, or yeasty origin or 1 × 107 cells of bacterial origin
[1] Fresh tissues or tissues stored in RNAlaterTM Stabilization Solution (Cat. No. AM7020).
  • Freshly prepared DNaseI solution included in the kit
Check Reagent 1 reaction (µL) _ _ _ _ reaction (µL) Memo
ddH2O (RNAse free) 87.5
10x DNase Buffer 10
DNaseI 2.5 12 units / µL
Total volume (µL) 100 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _









Protocol

1 Sample preparation
First step of sample preparation differs if you are using (A)culture cells OR (B)Tissue specimen. 
Buffers are highlighted in blue to prevent mistakes.
  • interactome/trizol.1575512694.txt.gz
  • Last modified: 2019/12/05 11:24
  • by dan